| Sıra | DOSYA ADI | Format | Bağlantı |
|---|---|---|---|
| 01. | Stages Fermentation Conferences Science | pptx | Sunumu İndir |
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A Metabolomics Approach to Study Chardonnay Wine Fermentation Chandra Richter, PhDE&J Gallo Winery MetabolomicsMarch 24, 20143
Winemaking at the Molecular Level4AAAAAAAAAO OROH OHRSGlu
WinemakingWaterSugarAcidsMinor ComponentsWaterEthanolGlycerolAcidsMinor Components
Yeast MetabolismSugarPyruvic AcidAcetaldehydeEthanolGlycerolDiacetylTCA CycleAcetic Acid Acetyl CoAFatty Acid CoAFatty AcidsMonoterpenesSulfateSulfiteAmino AcidsH2S MercaptansKeto AcidsAldehydesHigher AlcoholsEstersGlycosides Aglycones Cysteines ConjugatesVolatile Thiols
Identify metabolites present at three stages during fermentation7PDMTime (days)0 2 4 6 8 10RS (g/100ml)051015202530% Alcohol0246810121416Cell Concentration (MM cells/ml)020406080100120140RS Alcohol Cell Concentration Chardonnay JuiceGC-MS, LC-MS-MSLook for metabolic patterns
Winemaking Process• Chardonnay juice• 10 gallon• 65F• Agitation• PDM yeast• Triplicate• Sampled Daily8
Fermentation Timecourse9
Endo- and Exo-Metabolome10Exo-MetabolomeEndo-Metabolome
Summary of Metabolites11
PCA Separates Time12ABC
Glycoloysis13• Early stages of fermentation: sugars are high in the juice and low in the cell.• Cells are rapidly producing biomass.• Later stages in fermentation: cells reduce uptake of sugar and glycolysis slows.
TCA14• Similar pattern seen in the TCA cycle intermediates.• a-ketogluterate shows a unique profile• Deamination of glutamine
15Correlation ProfilesEndometabolome Exometabolome
Hierarchichal Relationship16Endometabolome Exometabolome
17Metabolic FluxEndometabolome Exometabolome
SugarPyruvic AcidAcetaldehydeEthanolGlycerolTCA CycleAcetic Acid Acetyl CoAFatty AcidsAmino AcidsKeto AcidsAldehydesHigher AlcoholsEstersRegulation of Metabolism
Conclusions• Identified the endo- and exo-metabolome over the course of a wine fermentation• Metabolic pathways are differentially regulated through fermentation– Glycolysis, TCA active during early stages– Amino acid catabolism increases activity at later stages• Provides understanding of key primary and secondary metabolites19
Acknowledgements • Dave Santino• Steven Kukesh• Nick Dokoozlian• Metabolon– Adam Kennedy– Lining Guo20
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Stages Fermentation Conferences Science
Yıl : 2020
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